Skin Biology Lab research topics.

	Staff member scientist: van Erp, Piet E.J. Ph.D/ senior scientist. March 24, 1956. Duties: 0.9 research, 0.1 teaching Tel. +31.24.3613548 Fax. +31.24.3541184 E-mail p.vanerp@derma.umcn.nl

					Education 1986 - 1991 University Hospital Nijmegen, Department of Dermatology, Ph.D. project entitled “Novel cytometric techniques to study proliferation and differentiation of human keratinocytes”, promotores: Prof. Dr. P.D. Mier and Prof. Dr. R. Happle ( University of Marburg , Germany ), co-promotores: Dr. J. Schalkwijk and Dr. P.C.M. van de Kerkhof 1973 - 1976 Laboratory School for Higher Professional Education Oss , B.Sc. in Biochemistry (HBO-B Biochemie) 1968 - 1973 High School Diploma (HAVO), Titus Brandsma lyceum Oss Professional Positions Student, 1976, Philips Eindhoven, Department of Plant Physiology, research project entitled “Relation between phenol metabolism and induction of flowering in plants” Technician, 1978 - 1982, University Hospital Nijmegen, Department of Dermatology, grant from Dutch Cancer Foundation (KWF), project entitled “Plasma membrane and epidermal proliferation” Research assistant and flow cytometry operator, 1983 - 1992, University Hospital Nijmegen, Department of Dermatology Research Scientist / Lecturer, 1993 - 2004, University Hospital Nijmegen , Department of Dermatology Senior Scientist, 2004 – present, Radboud University Medical Centre Nijmegen, Department of Dermatology – Skin Biology Laboratory Scientific Interest Epidermal growth and differentiation, Cytomics, Psoriasis, Tumor Cell Biology, Clinically oriented experimental Dermatology, skin-material interactions, product ergonomics in relation to skin Professional Associations European Society for Dermatological Research (ESDR) International Society for Analytical Cytometry (ISAC) European Society for Analytical Cellular Pathology (ESACP) Dutch Cell Biology Society Dutch Organization for Scientific Research (NWO) Dutch Dermatology and Venereology Society (NVDV) Dutch Society for Experimental Dermatology (NVED, 1999-2002 treasurer) Teaching experience 2000 – 2002: Participated in Education Module KMP1 “Genetic Skin Disorders” for Medical students 1996 - present: Participated in Education Module 2T8, presently TP02 “Immunopathology and Immunotoxicology” for students Biomedical Sciences 1995: Organizer of AIO Course “Analytical and Preparative Flow Cytometry” 1994 - 1995: Participated in AIO Course “Introduction of Biomolecules in Eukaryotic Cells” 1994 - present: Participated in ICS/NCMLS AIO Graduate Course “Advances in Cellular Signaling, Theoretical and Practical Approaches” 1994 - present: Organizer of DNA Flow cytometry Workshop for Dutch Coulter Flow Cytometer Users 1993 – 2000: Incidental participation in ALCO (Dermatology Research Overview for Medical Students at Dermatology) 1983 - present: (Master's) Research Supervision of Science and Medical students Thesis Supervision and Co-promotor 2004 – present: Immunofluorescent Characterization of Epidermal Growth and Differentiation – John Korver 2003 - present: Application of fluorescence diagnosis (FD) and photodynamic therapy (PDT) in psoriasis and cutaneous squamous premalignancies of the skin - Tim Smits 2003 – present: Peripheral Blood Mononuclear Cells and Therapy Modalities in Psoriasis – Annechien Langewouters 2003 – present: Infiltrating T-Cells in the Psoriatic Epidermis – Jorn Bovenschen 2003 - present: Cytometric Characterization of Epidermal Growth and Differentiation – Mirella van Duijnhoven 2002 - present: Immunocytes in the Pathogenesis of Psoriasis – Wynand Vissers 2000 - 2004: The Role of Transit Amplifying Cells in the Pathogenesis and Therapy of Psoriasis – Manon Franssen (grant NWO, 920-03-174)* 1997 - 2003: Phenotypical and Functional Characterization of Germinative Cell Populations in Human Epidermis – Michelle van Rossum (grant NWO, 920-03-099)* 1997 - 2000: Emergent Epidermal Phenotyping in Psoriasis – Roland Mommers 1996 - 2000: The Morphology of Proliferation in Psoriasis – Fransje Castelijns 1995 - 2000: Modulation of Psoriasis-specific Gene Expression in Human Keratinocytes - Miriam Wingens (grant NWO, 902-11-058)* 1994 - 1997: The Topical Treatment in Psoriasis; Immunohistochemical and Clinical Aspects - Carine van der Vleuten 1994 - 1997: Multiparameter Flow Cytometry as a Tool to Study Psoriasis - Conrad P. Glade 1994 - 1997: The Relevance of CD11b and CD18 in the Pathogenesis and Treatment of Psoriasis – Han van Pelt 1993 - 1998: Growth and Differentiation of Normal and Psoriatic Keratinocytes in vitro - Fred van Ruissen 1992 - 1995: Dynamics of Epidermal Growth and Keratinization in Psoriasis - Rianne Gerrits 1989 - 1993: Dynamics of Epidermal Growth and Inflammation in Psoriasis - Elke de Jong 1987 - 1991: Proliferation and Keratinization in Psoriasis - Saskia de Mare * grant owner and project leader, otherwise co-supervisor University Service Coordinator and responsible for laboratory management of a flow cytometry service for the Medical and Science Faculty in Nijmegen Miscellaneous Ad hoc reviewer for: The Journal of Investigative Dermatology Acta Dermato Venereologica Archives of Dermatological Research British Journal of Dermatology Cytometry Histochemical Journal Analytical Cellular Pathology



Publications

Book chapters van Erp, P.E.J. Proliferation, Differentiation and Inflammation in Normal and Hyperproliferative Skin Using Multiparameter Flow Cytometry. In: Epidermal Cell Protocols, edited by Turksen, K. and Walker, J. Totowa: Humana Press, 2004, p. 239- 250 van de Kerkhof, P.C.M. and van Erp, P.E.J. Pathogenesis. In: Textbook of psoriasis , edited by van de Kerkhof, P.C.M. Oxford: Blackwell Publishing, 2003, p. 93- 109 Brons, P.P., van Erp, P.E. and Pennings, A.H. Simultaneous DNA content and cell-surface immunofluorescence analysis. In: Flow Cytometry, part A , edited by Darzynkiewicz, Z., Robinson, J.P. and Crissman, H.A. San Diego : Academic Press, 1994, p. 95-102. van Erp, P., Jansen, M., de Jongh, G., Boezeman, J. and Schalkwijk, J. Flow cytometric measurement of intracellular pH in cultured human keratinocytes using carboxy-SNARF-1. In: Advances in Analytical Cellular Pathology , edited by Burger, G., Oberholzer, M. and Vooijs, G.P. Amsterdam: Excerpta Medica, 1990, p. 131-133. van Erp, P.E.J. and Mier, P.D. Molecular biology. In: Textbook of psoriasis , edited by Mier, P.D. and van de Kerkhof, P.C.M. Edinburgh: Churchill Livingstone, 1986, p. 125-149. Refereed publications Franssen ME, Zeeuwen PL, Vierwinden G, van de Kerkhof PC, Schalkwijk J, van Erp PE (2005) Phenotypical and functional differences in germinative subpopulations derived from normal and psoriatic epidermis. J.Invest Dermatol. 124, 373-383. van Duijnhoven MW, van de Kerkhof PC, Pasch MC, Muys L, van Erp PE (2005) The combination of the Zenon labeling technique and microscopic image analysis to study cell populations in normal and psoriatic epidermis. J.Cutan.Pathol. 32, 212-219. Vissers WH, Roelofzen J, De Jong EM, van Erp PE, van de Kerkhof PC (2005) Flexural versus plaque lesions in psoriasis: an immunohistochemical differentiation. Eur.J.Dermatol. 15, 13-17. Franssen ME, de Jongh GJ, van Erp PE , van de Kerkhof PC (2004) A left/right comparison of twice-daily calcipotriol ointment and calcitriol ointment in patients with psoriasis: the effect on keratinocyte subpopulations. Acta Derm.Venereol. 84, 195-200. Franssen ME, Boezeman JB, van de Kerkhof PC, van Erp PE (2004) Monitoring hyperproliferative disorders in human skin: flow cytometry of changing cytokeratin expression. Cytometry 57B, 32-39. van Erp PE (2004) Proliferation, differentiation, and inflammation in normal and hyperproliferative skin using multiparameter flow cytometry. In 'Epidermal Cells - Methods and Protocols'. (Ed. K Turksen) pp. 239-250. (Humana Press: Totowa , New Jersey ) van Rossum MM, Franssen MEJ, Cloin WAH, van de Bosch GJM, Boezeman JBM, Schalkwijk J, Van de Kerkhof PCM, Van Erp PEJ (2004) Functional characterization of beta 1-integrin-positive epidermal cell populations. Acta Dermato-Venereologica 84, 265-270. Vissers WH, Arndtz CH, Muys L, van Erp PE, De Jong EM, van de Kerkhof PC (2004) Memory effector (CD45RO+) and cytotoxic (CD8+) T cells appear early in the margin zone of spreading psoriatic lesions in contrast to cells expressing natural killer receptors, which appear late. Br.J Dermatol 150, 852-859. Vissers WH, Berends M, Muys L, van Erp PE, De Jong EM, van de Kerkhof PC (2004) The effect of the combination of calcipotriol and betamethasone dipropionate versus both monotherapies on epidermal proliferation, keratinization and T-cell subsets in chronic plaque psoriasis. Exp.Dermatol. 13, 106-112. Vissers WHPM, Muys L, Van Erp PEJ, De Jong EMGJ, Van de Kerkhof PCM (2004) Immunohistochemical differentiation between inflammatory linear verrucous epidermal nevus (ILVEN) and psoriasis. European Journal of Dermatology 14, 216-220. Franssen ME, Smit JV, van Erp PE , van de Kerkhof PC (2003) A multiparameter flow cytometric analysis of the effect of bexarotene on the epidermis of the psoriatic lesion. Br.J Dermatol 149, 506-512. Jansen BJ, Van Ruissen F, Cerneus S, Cloin W, Bergers M, van Erp PE, Schalkwijk J (2003) Tumor necrosis factor related apoptosis inducing ligand triggers apoptosis in dividing but not in differentiating human epidermal keratinocytes. J.Invest Dermatol. 121, 1433-1439. Van de Kerkhof PCM, Van Erp PEJ (2003) Pathogenesis. In 'Textbook of Psoriasis'. (Ed. PCM Van de Kerkhof) pp. 93-109. (Blackwell Publishing: Van Erp PEJ (2003) De huid als barriere. Cahiers Bio-Wetenschappen en Maatschappij 22, 3-8. Glade CP, van der Vleuten CJ, van Erp PE, van de Kerkhof PC (2002) Flow cytometric assessment of clearance and relapse characteristics in psoriasis vulgaris after treatment with weekly clobetasol lotion under hydrocolloid occlusion versus twice-daily clobetasol ointment. Skin Pharmacol Appl Skin Physiol 15, 92-9. Van Erp, P. E. J., van Rossum, M. M., Boezeman, J. B. M., Schalkwijk, J., and Van de Kerkhof, P. C. M. Clonal analysis of epidermal cells derived from small human skin punch biopsies. Journal of Investigative Dermatology 119[3], 748. 2002. Ref Type: Abstract van Rossum MM, Schalkwijk J, van de Kerkhof PC, van Erp PE (2002) Immunofluorescent surface labelling, flow sorting and culturing of putative epidermal stem cells derived from small skin punch biopsies. J Immunol Methods 267, 109-17. Franssen, M. E. J., Smit, J. V., Van Erp, P. E. J., and Van de Kerkhof, P. C. M. A multiparameter flow cytometric analysis of the effect of bexarotene on the epidermis of the psoriatic lesion. Journal of Investigative Dermatology 117[3], 817. 2001. Ref Type: Abstract van Erp PE, Wingens M (2001) Does antisense make sense in dermatology? Acta Derm Venereol 81, 385-91. van Rossum MM, Cloin WAH, Franssen MEJ, Van de Kerkhof PCM, Van Erp PEJ (2001) Identification of epidermal stem cells with a combination of flow cytometry, cell culture and image processing. Journal of Investigative Dermatology 117, 787. van Rossum MM, van Erp PE, van de Kerkhof PC (2001) Treatment of psoriasis with a new combination of calcipotriol and betamethasone dipropionate: a flow cytometric study. Dermatology 203, 148-52. Castelijns FA, Gerritsen MJ, van Erp PE, van de Kerkhof PC (2000) Cell-kinetic evidence for increased recruitment of cycling epidermal cells in psoriasis: the ratio of histone and Ki-67 antigen expression is constant. Dermatology 201, 105-10. Castelijns FA, Gerritsen MJ, van Erp PE , van de Kerkhof PC (2000) Efficacy of calcipotriol ointment applied under hydrocolloid occlusion in psoriasis. Dermatology 200, 25-30. Emri G, Wenczl E, Van Erp P, Jans J, Roza L, Schothorst AA, Horkay I (2000) UV-irradiation results in delay of cell cycle progression and formation of micronuclei with different effectiveness in cultured human fibroblasts and melanocytes as determined by flow cytometry. Cytometry 42, 146-147. Emri G, Wenczl E, Van Erp P, Jans J, Roza L, Horkay I, Schothorst AA (2000) Low doses of UVB or UVA induce chromosomal aberrations in cultured human skin cells. J Invest Dermatol 115, 435-40. Emri G, Wenczl E, Van Erp P, Jans J, Roza L, Schothorst AA, Horkay I (2000) UV-irradiation results in delay of cell cycle progression and formation of micronuclei with different effectiveness in cultured human fibroblasts and melanocytes as determined by flow cytometry. Cytometry 42, 146-147. Franssen MEJ, Mommers JM, Cloin WAH, van Rossum MM, Van de Kerkhof PCM, Van Erp PEJ (2000) Monitoring psoriasis: an improved multiparameter flow cytometric assay. Journal of Investigative Dermatology 115, 582. Mommers JM, van Rossum MM, van Erp PE, van de Kerkhof PC (2000) Changes in keratin 6 and keratin 10 (co-)expression in lesional and symptomless skin of spreading psoriasis. Dermatology 201, 15-20. Mommers JM, Goossen JW, van de Kerkhof PC, van Erp PE (2000) Novel functional multiparameter flow cytometric assay to characterize proliferation in skin. Cytometry 42, 43-9. Mommers JM, Van Erp PEJ, Van de Kerkhof PCM (2000) No clinical effect of a specific LTB4 receptor antagonist (VML295) in the treatment of psoriasis. Journal of Investigative Dermatology 115, 590. Seegers BA, Andriessen MP, van Hooijdonk CA, De Bakker ES, van Vlijmen-Willems IM, Parker GL, van Erp PE, van de Kerkhof PC (2000) Pharmacological effects of a specific leukotriene B(4) receptor antagonist (VML 295) on blood leukocytes, cutaneous inflammation and epidermal proliferation. Skin Pharmacol Appl Skin Physiol 13, 75-85. Seegers BA, Glade CP, van Hooijdonk CA, van Erp PE, de Jongh GJ, van de Kerkhof PC (2000) Flow-cytometric characterization of normal versus psoriatic epidermis using improved cell separation methodology. Exp Dermatol 9, 266-70. Van Erp PEJ, Mommers JM, Castelijns FACM, Vlijmen-Willems IMJJ, Van de Kerkhof PCM (2000) Relapse of psoriatic lesions as an in vivo model to study early proliferation-associated events. Journal of Investigative Dermatology 115, 583. van Rossum MM, Mommers JM, van de Kerkhof PC, van Erp PE (2000) Coexpression of keratins 13 and 16 in human keratinocytes indicates association between hyperproliferation-associated and retinoid-induced differentiation. Arch Dermatol Res 292, 16-20. van Rossum MM, Mommers JM, van Erp PE, Leyninger E, Clucas A, van de Kerkhof PC (2000) CD 2394, a novel synthetic retinoid, initiates an embryonic type of differentiation in hyperproliferative skin. Acta Derm Venereol 80, 98-101. van Rossum MM, Van de Kerkhof PCM, Cloin WAH, Fransen MEJ, Van Erp PEJ (2000) Characterisation of epidermal stem cells: Strategy and methodology. Journal of Investigative Dermatology 115, 545. Castelijns FA, Gerritsen MJ, van Vlijmen-Willems IM, van Erp PJ, van de Kerkhof PC (1999) Proliferation is the main epidermal target in the treatment of psoriatic plaques with once daily application of tacalcitol ointment. Acta Derm Venereol 79, 111-4. Castelijns FA, Gerritsen MJ, van Erp PE, van de Kerkhof PC (1999) Immunohistochemical assessment of keratin 16 on paraffin-embedded sections of normal and hyperproliferative skin: monoclonal antibodies Ks8.12 and LL025 in a comparative study. Arch Dermatol Res 291, 59-63. Castelijns FA, Gerritsen MJ, van Vlijmen-Willems IM, van Erp PE, van de Kerkhof PC (1999) The epidermal phenotype during initiation of the psoriatic lesion in the symptomless margin of relapsing psoriasis. J Am Acad Dermatol 40, 901-9. Gerritsen MJ, van Erp PE , van de Kerkhof PC (1999) Plasma membrane bound transglutaminase in the marginal zone of psoriatic skin. Clin Exp Dermatol 24, 237-9. Mommers JM, ter Meulen AC, van Erp PE, van de Kerkhof PC (1999) Influence of tacalcitol on cell cycle kinetics of human keratinocytes following standardized injury. Skin Pharmacol Appl Skin Physiol 12, 174-81. Mommers JM, van Erp PE , van de Kerkhof PC (1999) Clobetasol under hydrocolloid occlusion in psoriasis results in a complete block of proliferation and in a rebound of lesions following discontinuation. Dermatology 199, 323-7. Mommers JM, van Rossum MM, van Hooijdonk CA, van Erp PE, van de Kerkhof PC (1999) Quantification of epidermal cell populations in the centre and margin of stable psoriatic plaques. Arch Dermatol Res 291, 88-92. van Rossum MM, Mommers JM, van Hooijdonk CA, van Erp PE, van de Kerkhof PC (1999) The response of distant uninvolved psoriatic skin to standardised injury is not different from that in normal skin. Skin Pharmacol Appl Skin Physiol 12, 271-5. Wingens M, Pfundt R, van Vlijmen-Willems IM, van Hooijdonk CA, van Erp PE, Schalkwijk J (1999) Sequence-specific inhibition of gene expression in intact human skin by epicutaneous application of chimeric antisense oligodeoxynucleotides. Lab Invest 79, 1415-24. Castelijns F, Gerritsen MJ, Van Erp P, van de Kerkhof P (1998) Immunohistochemical assessment of keratin 16 on paraffin embedded sections of normal and hyperproliferative skin: Monoclonal antibodies Ks8.12 and LL025 in a comparative study. Journal of Investigative Dermatology 110, 560. Castelijns FA, Ezendam J, Latijnhouwers MA, van Vlijmen-Willems IM, Zeeuwin PL, Gerritsen MJ, van de Kerkhof PC, van Erp PE (1998) Epidermal cell kinetics by combining in situ hybridization and immunohistochemistry. Histochem J 30, 869-77. Gerritsen MJ, Castelijns FACM, Van Erp PEJ, Van de Kerkhof PCM (1998) The use of clobetasol-17-propionate under hydrocolloid dressing in a psoriasis clearance-relapse model: A new approach. Journal of Investigative Dermatology 110, 560. Glade CP, van Erp PE, Werner-Schlenzka H, van de Kerkhof PC (1998) A clinical and flow cytometric model to study remission and relapse in psoriasis. Acta Derm Venereol 78, 180-5. Glade CP, van der Vleuten CJ, van Erp PE, De Jong EM, van de Kerkhof PC (1998) The epidermis of chronic idiopathic lichen planus during topical treatment with the vitamin D3 analogue KH 1060. Clin Exp Dermatol 23, 14-8. Lahaye DH, Camps MG, van Erp PE, Peters PH, Zoelen EJ (1998) Epidermal growth factor (EGF) receptor density controls mitogenic activation of normal rat kidney (NRK) cells by EGF. J Cell Physiol 174, 9-17. Mommers JM, Castelijns FA, Seegers BA, van Rossum MM, van Hooijdonk CA, van Erp PE, van de Kerkhof PC (1998) The effect of long-term treatment with tacalcitol on the psoriatic epidermis. A flow cytometric analysis. Br J Dermatol 139, 468-71. Mommers JM, Castellins FACM, Seegers BAMP, van Rossum MM, van Hooijdonk CAEM, Van Erp PEJ, Van de Kerkhof PCM (1998) Flow cytometrical analysis of the psoriatic epidermis after long-term treatment with 1 alpha,24-dihydroxyvitamin D-2 (tacalcitol). Journal of Investigative Dermatology 110, 683. Van Pelt JP, De Jong EM, Seijger MM, van Hooijdonk CA, De Bakker ES, Van Vlijmen IM, Parker GL, van Erp PE, van de Kerkhof PC (1998) Investigation on a novel and specific leukotriene B4 receptor antagonist in the treatment of stable plaque psoriasis. Br J Dermatol 139, 396-402. van Rossum MM, Mommers JM, van Hooijdonk CAEM, Van Erp PEJ, Van de Kerkhof PCM (1998) The response of distant uninvolved psoriatic skin and normal skin to standardised injury. Journal of Investigative Dermatology 110, 557. van Rossum MM, Mommers JM, van de Kerkhof PC, de Jongh GJ, van Hooijdonk CA, van Erp PE (1998) Multi parameter flow cytometric assessment of regenerative epidermis with special reference to the antiproliferative effect of occlusion. Anal Cell Pathol 17, 157-65. Wingens M, van Hooijdonk CA, de Jongh GJ, Schalkwijk J, van Erp PE (1998) Flow cytometric and microscopic characterization of the uptake and distribution of phosphorothioate oligonucleotides in human keratinocytes. Arch Dermatol Res 290, 119-25. Glade CP, van Erp PE, Boezeman JB, van de Kerkhof PC (1997) Multiparameter flow cytometry as a tool to evaluate antipsoriatic therapy. Br J Dermatol 137, 367-75. van Hooijdonk CA, Colbers RM, Piek J, van Erp PE (1997) Demonstration of an Na+/H+ exchanger in mouse keratinocytes measured by the novel pH-sensitive fluorochrome SNARF-calcein. Cell Prolif 30, 351-63. Van Pelt JP, De Jong EM, van Erp PE, Mitchell MI, Marder P, Spaethe SM, van Hooijdonk CA, Kuijpers AL, van de Kerkhof PC (1997) The regulation of CD11b integrin levels on human blood leukocytes and leukotriene B4-stimulated skin by a specific leukotriene B4 receptor antagonist (LY293111). Biochem Pharmacol 53, 1005-12. vanPelt JPA, Dejong EMGJ, Vanerp PEJ, VandeKerkhof PCM (1997) Decreased CD11b expression on circulating polymorphonuclear leukocytes in patients with extensive plaque psoriasis. European Journal of Dermatology 7, 324-328. Wingens M, vanHooijdonk CAEM, Schalkwijk J, Vanerp PEJ (1997) Modulation of psoriasis-related gene expression by antisense oligonucleotides. Journal of Investigative Dermatology 109, 443. Gerritsen MJP, Vanerp PEJ, VandeKerkhof PCM (1996) Transglutaminase expression in lesional skin, distant clinically uninvolved skin, and in the margin zone of psoriasis. Journal of Investigative Dermatology 107, 100. Glade CP, Seegers BA, Meulen EF, van Hooijdonk CA, van Erp PE, van de Kerkhof PC (1996) Multiparameter flow cytometric characterization of epidermal cell suspensions prepared from normal and hyperproliferative human skin using an optimized thermolysin-trypsin protocol. Arch Dermatol Res 288, 203-10. Glade CP, Vanerp PEJ, VandeKerkhof PCM (1996) Treatment of psoriasis vulgaris with calcipotriol cream and topical corticosteroids: A comparative flow cytometric study. Journal of Investigative Dermatology 107, 107. Glade CP, van Erp PE, van de Kerkhof PC (1996) Epidermal cell DNA content and intermediate filaments keratin 10 and vimentin after treatment of psoriasis with calcipotriol cream once daily, twice daily and in combination with clobetasone 17-butyrate cream or betamethasone 17-valerate cream: a comparative flow cytometric study. Br J Dermatol 135, 379-84. Pfundt R, Van Ruissen F, van Vlijmen-Willems IM, Alkemade HA, Zeeuwen PL , Jap PH, Dijkman H, Fransen J, Croes H, van Erp PE, Schalkwijk J (1996) Constitutive and inducible expression of SKALP/elafin provides anti-elastase defense in human epithelia. J Clin Invest 98, 1389-99. van de Kerkhof PC, van Erp PE (1996) The role of epidermal proliferation in the pathogenesis of psoriasis. Skin Pharmacol 9, 343-54. van Erp PE, Boezeman JB, Brons PP (1996) Cell cycle kinetics in normal human skin by in vivo administration of iododeoxyuridine and application of a differentiation marker--implications for cell cycle kinetics in psoriatic skin. Anal Cell Pathol 11, 43-54. Van Ruissen F, de Jongh GJ, van Erp PE, Boezeman JB, Schalkwijk J (1996) Cell kinetic characterization of cultured human keratinocytes from normal and psoriatic individuals. J Cell Physiol 168, 684-94. Van Ruissen F, de Jongh GJ, Zeeuwen PL , van Erp PE, Madsen P, Schalkwijk J (1996) Induction of normal and psoriatic phenotypes in submerged keratinocyte cultures. J Cell Physiol 168, 442-52. vanRuissen F, deJongh GJ, Zeeuwen LJM, Vanerp PEJ, Madsen P, Schalkwijk J (1996) In vitro induction of normal and psoriatic phenotypes. Journal of Investigative Dermatology 107, 102. Wingens M, vanHooijdonk CAEM, deJongh GJ, Schalkwijk J, Vanerp PEJ (1996) Uptake and intracellular distribution of oligonucleotides in cultured human keratinocytes. Journal of Investigative Dermatology 107, 10. Gerritsen MJ, van Erp PE, van de Kerkhof PC (1995) Transglutaminase-positive cells in psoriatic epidermis during treatment with calcitriol (1alpha,25 dihydroxy vitamin D3) and tacalcitol (1alpha,24 dihydroxy vitamin D3). Br J Dermatol 133, 656-9. Glade CP, van Erp PE, van Hooijdonk CA, Elbers ME, van de Kerkhof PC (1995) Topical treatment of psoriatic plaques with 1 alpha, 24 dihydroxyvitamin D3: a multiparameter flow cytometrical analysis of epidermal growth, differentiation and inflammation. Acta Derm Venereol 75, 381-5. Glade CP, Botermans RJ, van Erp PE, van de Kerkhof PC (1995) The dynamics of the response of normal skin to single and multiple epicutaneous leukotriene B4 applications analysed by three-colour flow cytometry. Acta Derm Venereol 75, 437-41. van de Kerkhof PC, De Mare S, Arnold WP, van Erp PE (1995) Epidermal regeneration and occlusion. Acta Derm Venereol 75, 6-8. Vanerp PEJ, Hegeman GM, vanHooijdonk CAEM (1995) A Flow-Cytometric Characterization of Hacat Keratinocytes - A Model for Epidermal Growth-Regulation. Journal of Investigative Dermatology 105, 466. vanHooijdonk CAEM, Glade CP, Seegers BAMP, Meulen EFJ, Vanerp PEJ, VandeKerkhof PCM (1995) 3-Color Flow-Cytometric Analysis of Epidermal-Cell Suspensions Prepared from Normal and Hyperproliferative Human Skin Using An Optimized Thermolysin-Trypsin Separation Method. Journal of Investigative Dermatology 105, 496. vanPelt JPA, Dejong EMGJ, Vanerp PEJ, VandeKerkhof PCM (1995) Leukocyte Integrin Expression and Their Migration in Inflamed Skin, Effects of A Novel Ltb(4) Receptor Antagonist. Journal of Investigative Dermatology 105, 465. Alkemade JA, Molhuizen HO, Ponec M, Kempenaar JA, Zeeuwen PL, de Jongh GJ, van Vlijmen-Willems IM, van Erp PE, van de Kerkhof PC, Schalkwijk J (1994) SKALP/elafin is an inducible proteinase inhibitor in human epidermal keratinocytes. J Cell Sci 107 ( Pt 8), 2335-42. Botermans RJG, Glade CP, Vanerp PEJ, VandeKerkhof PCM (1994) A Multiparameter Flow Cytometric Study of the Response of Normal Skin to Single and Multiple Topical Application of Ltb(4). Journal of Investigative Dermatology 103, 404. Brons PP, van Erp PE , Pennings AH (1994) Simultaneous DNA content and cell-surface immunofluorescence analysis. Methods Cell Biol 41, 95-102. Gerritsen MJ, van Erp PE, van Vlijmen-Willems IM, Lenders LT, van de Kerkhof PC (1994) Repeated tape stripping of normal skin: a histological assessment and comparison with events seen in psoriasis. Arch Dermatol Res 286, 455-61. Glade CP, Vanerp PEJ, vanHooijdonk CAEM, Elbers ME, VandeKerkhof PCM (1994) Treatment of Psoriasis with 1-Alpha,24 Dihydroxyvitamin D-3 - A Flow Cytometric Multiparameter Study. Journal of Investigative Dermatology 103, 431. Jensen PO, Larsen JK, Christensen IJ, van Erp PE (1994) Discrimination of bromodeoxyuridine labelled and unlabelled mitotic cells in flow cytometric bromodeoxyuridine/DNA analysis. Cytometry 15, 154-61. van der Ven AJ, Mier P, Peters WH, Dolstra H, van Erp PE, Koopmans PP, van der Meer JW (1994) Monochlorobimane does not selectively label glutathione in peripheral blood mononuclear cells. Anal Biochem 217, 41-7. van Erp PE, de Jongh GJ, Boezeman JB, Schalkwijk J (1994) The growth and differentiation of human keratinocytes in vitro: a combined immunohistochemical and flow cytometric study. Arch Dermatol Res 286, 115-22. van Hooijdonk CA, Glade CP, van Erp PE (1994) TO-PRO-3 iodide: a novel HeNe laser-excitable DNA stain as an alternative for propidium iodide in multiparameter flow cytometry. Cytometry 17, 185-9. Van Ruissen F, van Erp PE, de Jongh GJ, Boezeman JB, van de Kerkhof PC, Schalkwijk J (1994) Cell kinetic characterization of growth arrest in cultured human keratinocytes. J Cell Sci 107 ( Pt 8), 2219-28. Vanerp PEJ, Piek J, vanHooijdonk CAEM (1994) Flow Cytometric Measurement of Ph(I) in Keratinocytes - Discrimination Between Quiescent and Proliferative Cells. Journal of Investigative Dermatology 103, 431. vanRuissen F, Vanerp PEJ, deJongh GJ, Boezeman JBM, VandeKerkhof PCM, Schalkwijk J (1994) Cell Kinetic Characterization of Growth Arrest in Cultured Human Keratinocytes. Journal of Investigative Dermatology 103, 399. Gerritsen MJ, Rulo HF, van Vlijmen-Willems I, van Erp PE, van de Kerkhof PC (1993) Topical treatment of psoriatic plaques with 1,25-dihydroxyvitamin D3: a cell biological study. Br J Dermatol 128, 666-73. Mier PD, van Hooijdonk CA , Freund RF, de Jongh GJ, van Erp PE, van de Kerkhof PC (1993) EGF induces ornithine decarboxylase transcription in Balb/MK keratinocytes: a proposed mechanism for the TGF alpha autocrine loop. Arch Dermatol Res 285, 236-8. Suykerbuyk EJA, Schalkwijk J, Vanerp PEJ (1993) Invivo Expression of Proliferating Cell Nuclear Antigen in Human Keratinocytes Following Standardized Injury. Journal of Investigative Dermatology 100, 454. van Hooijdonk CA, van Erp PE, Freund RL, de Jongh GJ, Schalkwijk J, van de Kerkhof PC, Mier PD (1993) Cell kinetic characterization of the epidermal growth factor dependent BALB/MK line using flow cytometric analysis of DNA content and iododeoxyuridine incorporation. Cell Prolif 26, 567-76. van Vlijmen-Willems I, Van Erp P (1993) Microwave irradiation for rapid and enhanced immunohistochemical staining: application to skin antigens. Biotech Histochem 68, 67-74. Vanerp PEJ, Suykerbuyk EJA, deJongh GJ, Schalkwijk J (1993) Cell Kinetic Characterization of Keratinocyte Growth and Differentiation Invitro. Journal of Investigative Dermatology 100, 464. vanHooijdonk CAEM, Vanerp PEJ, Freund RL, deJongh GJ, Mier PD (1993) A Cell Kinetic and Biochemical-Characterization of the Balb Mk Cell-Line. Journal of Investigative Dermatology 100, 455. Brons PP, Raemaekers JM, Bogman MJ, van Erp PE, Boezeman JB, Pennings AH, Wessels HM, Haanen C (1992) Cell cycle kinetics in malignant lymphoma studied with in vivo iododeoxyuridine administration, nuclear Ki-67 staining, and flow cytometry. Blood 80, 2336-43. De Jong EM, van Erp PE , Van Vlijmen IM, van de Kerkhof PC (1992) The inter-relation between inflammation and epidermal proliferation in normal skin following epicutaneous application of leukotriene-B4--an immunohistochemical study. Clin Exp Dermatol 17, 413-20. Rijzewijk JJ, Groenendal H, Van Erp P, Bauer FW, van Vloten WA (1992) Cell kinetics in skin disorders with disturbed keratinization. Acta Derm Venereol 72, 256-8. van der Steen PH, van Baar HM, Boezeman JB, van Erp PE, Van Vlijmen IM, van de Kerkhof PC (1992) Epidermal proliferation and keratinization following standardized elicitation with diphenylcyclopropenone. Clin Exp Dermatol 17, 316-20. van Duijnhoven JL, Schalkwijk J, Kranenborg MH, van Vlijmen-Willems IM, Groeneveld A, van Erp PE, Timmer ED, de Jongh GJ, van de Ven WJ (1992) MON-150, a versatile monoclonal antibody against involucrin: characterization and applications. Arch Dermatol Res 284, 167-72. De Jong EM, van Erp PE, Ruiter DJ, van de Kerkhof PC (1991) Immunohistochemical detection of proliferation and differentiation in discoid lupus erythematosus. J Am Acad Dermatol 25, 1032-8. De Jong EM, Van Vlijmen IM, van Erp PE, Ramaekers FC, Troyanovski SM, van de Kerkhof PC (1991) Keratin 17: a useful marker in anti-psoriatic therapies. Arch Dermatol Res 283, 480-2. van Erp PE, Jansen MJ, de Jongh GJ, Boezeman JB, Schalkwijk J (1991) Ratiometric measurement of intracellular pH in cultured human keratinocytes using carboxy-SNARF-1 and flow cytometry. Cytometry 12, 127-32. Vanerp PEJ, Boezeman JBM, Brons PPT (1991) Cell-Cycle Kinetics in Normal Human Epidermis by Invivo Administration of Iododeoxyuridine and Application of the Differentiation Marker Rkse60. Journal of Investigative Dermatology 96, 1018. De Mare S, de Jong E, van Erp PE, van de Kerkhof PC (1990) Markers for proliferation and keratinization in the margin of the active psoriatic lesion. Br J Dermatol 122, 469-75. De Mare S, van Erp PE, Ramaekers FC, van de Kerkhof PC (1990) Flow cytometric quantification of human epidermal cells expressing keratin 16 in vivo after standardized trauma. Arch Dermatol Res 282, 126-30. Dejong EMGJ, Vanerp PEJ, Vanvlijmen IMJJ, VandeKerkhof PCM (1990) Leukotriene-B4-Induced Inflammation and Proliferation. Journal of Investigative Dermatology 95, 466. Demare S, Dejong EMGJ, Vanerp PEJ, VandeKerkhof PCM (1990) Markers for Proliferation and Keratinization in the Margin of the Active Psoriatic Lesion. Journal of Investigative Dermatology 95, 466. Jansen MJJM, Vanerp PEJ, deJongh GJ, Boezeman JBM, Schalkwijk J (1990) Flow Cytometric Measurement of Intracellular Ph in Cultured Human Keratinocytes. Journal of Investigative Dermatology 95, 474. Van Erp P, Jansen M, de Jongh GJ, Boezeman J, Schalkwijk J (1990) Flow cytometric measurement of intracellular pH in cultured human keratinocytes using carboxy-SNARF-1. In 'Advances in analytical cellular pathology'. (Eds G Burger, M Oberholzer, and GP Vooijs) pp. 131-133. (Excerpta Medica: Amsterdam ) Vanerp PEJ, Boezeman JBM, deJongh GJ, Schalkwijk J (1990) Comparison of Flow Cytometric Techniques to Study Epidermal Proliferation Invivo and Invitro. Journal of Investigative Dermatology 95, 494. Beurskens T, Chang A, van Erp PE, van de Kerkhof PC (1989) Epidermal proliferation and accumulation of polymorphonuclear leukocytes in the psoriatic lesion. Dermatologica 178, 67-72. De Mare S, van Erp PE, van de Kerkhof PC (1989) Epidermal hyperproliferation assessed by the monoclonal antibody Ks8.12 on frozen sections. J Invest Dermatol 92, 130-1. Demare S, Denhartog G, Calis N, Vanerp PEJ, VandeKerkhof PCM (1989) The Expression of Keratin-16 and Percentages of Cells in Sg2M Phase in Psoriatic Lesions Treated with Dithranol Creams. Journal of Investigative Dermatology 92, 476. Rijzewijk JJ, van Erp PE, Bauer FW (1989) Two binding sites for Ki67 related to quiescent and cycling cells in human epidermis. Acta Derm Venereol 69, 512-5. Steijlen PM, Hamm H, Vanerp PEJ, Johnson JP, Ruiter DJ, Brocker EB (1989) Immunohistologic Evidence for the Malignant Potential of Congenital Melanocytic Nevi. Journal of Investigative Dermatology 92, 366-370. van Erp PE , De Mare S, Rijzewijk JJ, van de Kerkhof PC, Bauer FW (1989) A sequential double immunoenzymic staining procedure to obtain cell kinetic information in normal and hyperproliferative epidermis. Histochem J 21, 343-7. van Erp PE, Rijzewijk JJ, Boezeman JB, Leenders J, De Mare S, Schalkwijk J, van de Kerkhof PC, Ramaekers FC, Bauer FW (1989) Flow cytometric analysis of epidermal subpopulations from normal and psoriatic skin using monoclonal antibodies against intermediate filaments. Am J Pathol 135, 865-70. Beurskens T, Chang A, Vanerp PEJ, Happle R, VandeKerkhof PCM (1988) Epidermal Proliferation and Accumulation of Polymorphonuclear Leukocytes in the Psoriatic Lesion. Journal of Investigative Dermatology 91, 411. De Mare S, Calis N, den Hartog G, van Erp PE, van de Kerkhof PC (1988) The relevance of salicylic acid in the treatment of plaque psoriasis with dithranol creams. Skin Pharmacol 1, 259-64. Demare S, Vanerp PEJ, Rijzewijk JJ, Boezeman JBM, VandeKerkhof PCM, Bauer FW (1988) A Sequential Double Immunoenzymatic Staining Procedure to Obtain Cell Kinetic Information in Normal and Hyperproliferative Epidermis. Journal of Investigative Dermatology 91, 376. van Bokhoven MG, De Mare S, Czarnetzki BM, van Erp PE, Boezeman JB, van de Kerkhof PC (1988) Active vitamin D3 does not suppress recruitment of G0 cells following injury. Br J Dermatol 119, 737-42. van Erp PE, van de Kerkhof PC (1988) Epidermal calmodulin levels and reference variables. J Invest Dermatol 90, 536-7. van Erp PE, Brons PP, Boezeman JB, de Jongh GJ, Bauer FW (1988) A rapid flow cytometric method for bivariate bromodeoxyuridine/DNA analysis using simultaneous proteolytic enzyme digestion and acid denaturation. Cytometry 9, 627-30. Vanbokhoven MGJ, Czarnetzki BM, Vanerp PEJ, Demare S, Happle R, VandeKerkhof PCM (1988) Topical Application of 1-Alpha,25-Dihydroxycholecalciferol Fails to Modulate Epidermal Growth-Invivo. Journal of Investigative Dermatology 91, 383. Vanbokhoven MGJ, Demare S, Czarnetzki BM, Vanerp PEJ, Boezeman JBM, VandeKerkhof PCM (1988) Active Vitamin-D3 Does Not Suppress Recruitment of G0 Cells Following Injury. British Journal of Dermatology 119, 737-742. VandeKerkhof PCM, Demare S, Vanerp PEJ (1988) Psoriatic and Trauma-Induced Hyperproliferation Assessed by the Monoclonal-Antibody Ks8.12 on Frozen-Sections. Journal of Investigative Dermatology 91, 377. Vanerp PEJ, Rijzewijk JJ, Leenders J, Ramaekers FCS, VandeKerkhof PCM, Bauer FW (1988) Simultaneous Intermediate Filament Dna Analysis of Epidermal-Cell Suspensions Derived from Normal and Psoriatic Skin Using Flow-Cytometry. Journal of Investigative Dermatology 91, 377. Bauer FW, Boezeman JBM, Vanerp PEJ, Rijzewijk JJ (1987) The Application of Bromodeoxyuridine for Kinetic-Studies in Epidermis. Journal of Investigative Dermatology 89, 312. Rijzewijk JJ, Groenendal H, Vanerp PEJ, Bauer FW (1987) 2 Antigenic Binding-Sites for Ki67 Related to Quiescent and Cycling Cells in Human-Epidermis. Journal of Investigative Dermatology 89, 313. van Erp PE, van de Kerkhof PC (1987) Calmodulin levels in psoriasis and other skin disorders. Arch Dermatol Res 279, 151-3. Vanerp PEJ, Groenendal H, Bauer FW, Rijzewijk JJ (1987) Growth Fraction in Epidermal Skin Disorders Determined by the Monoclonal-Antibody Ki67. Journal of Investigative Dermatology 89, 313. van der Meeren HL, van Erp PE (1986) Life-threatening contact urticaria from glove powder. Contact Dermatitis 14, 190-1. Van Erp PEJ, Mier PD (1986) Molecular biology. In 'Textbook of psoriasis'. (Eds PD Mier and PCM Van de Kerkhof) pp. 125-149. (Churchill Livingstone: Edinburgh ) Gommans JM, van Erp PE , Forster S, Boezeman J, Mier PD (1985) Isolation and preliminary biochemical characterization of the human epidermal Langerhans cell. J Invest Dermatol 85, 191-3. Roelfzema H, van Erp PE, Mier PD (1985) Glycoprotein metabolism in skin cancer: synthesis, pool size, and partial characterization of glycoproteins in the rat basosquamous cell carcinoma. J Invest Dermatol 85, 235-8. van de Kerkhof PC, van Erp PE (1985) Epidermal calmodulin and skin disease. Int J Dermatol 24, 507-8. Geerdink JP, de Mulder PH, Franzen MR, Gommans JM, van Rennes H, van Erp PE, Bergers M, Mier PD (1984) Monocyte function is normal in quiescent psoriasis. J Invest Dermatol 82, 122-5. Gommans JM, Vanerp PEJ, Forster S, Boezeman JBM, Mier PD (1984) Isolation and Preliminary Biochemical-Characterization of the Human Epidermal Langerhans Cell. Journal of Investigative Dermatology 82, 544. Mier PD, Vanerp PEJ, Roelfzema H (1984) Glycoprotein Metabolism in Epitheliomas. Journal of Investigative Dermatology 82, 566. van Erp PE , Bergers M, de Grood RM, Koedam J, Rijntjes JM (1984) Fucosyl glycopeptide profiles of keratinocytes from various epithelial tissues of the rabbit in relation to differentiation in vivo and in vitro. J Invest Dermatol 83, 359-62. VandeKerkhof PCM, Vanerp PEJ, Mier PD, Verhagen AR (1984) Calmodulin Levels in Psoriasis. Journal of Investigative Dermatology 82, 562. Vanerp PEJ, Bergers AJM, Degrood RM, Mier PD (1984) Epithelial Differentiation Is Related to Glycoprotein Composition. Journal of Investigative Dermatology 82, 548. Verhagen A, Bergers M, van Erp PE, Gommans JM, van de Kerkhof PC, Mier PD (1984) Confirmation of raised phospholipase A2 activity in the uninvolved skin of psoriasis. Br J Dermatol 110, 731-2. Feyen JH, van Erp PE, Mier PD (1983) Rapid technique for the radiometric assay of phospholipase A2. J Chromatogr 259, 338-40. Roelfzema H, van Erp PE (1983) Glycoprotein composition of psoriatic epidermis in relation to growth control. J Invest Dermatol 80, 20-3. Roelfzema H, van Erp PE (1983) Studies on the plasma membrane of normal and psoriatic keratinocytes. 6. Cell surface and shed glycoproteins. J Invest Dermatol 80, 24-6. van de Kerkhof PC, van Erp PE (1983) Calmodulin levels are grossly elevated in the psoriatic lesion. Br J Dermatol 108, 217-8. Gommans JM, van den Hurk JJ, Bergers M, Van Erp P, Mier PD, Roelfzema H (1982) Studies on the plasma membrane of normal and psoriatic keratinocytes. 5. Lectin binding. Br J Dermatol 106, 317-22. Mier PD, van Rennes H, van Erp PE, Roelfzema H (1982) Cutaneous sialidase. J Invest Dermatol 78, 267-9. Mier PD, van Rennes H, van Erp PE, Roelfzema H (1982) Alpha-l-fucosidase in normal and psoriatic epidermis. Br J Dermatol 106, 367-8. Roelfzema H, Bergers M, van Erp PE, Gommans JM, Mier PD (1981) Studies on the plasma membrane of normal and psoriatic keratinocytes. 3. Uptake of labelled sugars and their incorporation into glycoconjugates. Br J Dermatol 104, 635-40. Roelfzema H, Bergers M, van Erp PE, Gommans JM, Mier PD (1981) Studies on the plasma membrane of normal and psoriatic keratinocytes. 4. Characterization of glycoconjugates. Br J Dermatol 105, 509-16. Geerdink JP, Bergers M, van Erp PE , Gommans JM, Mier PD, Roelfzema H (1980) Cyclic AMP is decreased in mononuclear leukocytes from psoriasis patients. Br J Dermatol 103, 107-8. Gommans JM, Bergers M, van Erp PE, van den Hurk JJ, Mier PD, Roelfzema H (1979) Studies on the plasma membrane of normal and psoriatic keratinocytes. I. Preparation of material and morphological characterization. Br J Dermatol 101, 407-12. Gommans JM, Bergers M, van Erp PE, van den Hurk JJ, van de Kerkhof P, Mier PD, Roelfzema H (1979) Studies on the plasma membrane of normal and psoriatic keratinocytes. 2. Cyclic AMP and its response to hormonal stimulation. Br J Dermatol 101, 413-9.









Organisation I Staff I Research I Articles I Protocols & Facilities I NCMLS I Courses & Seminars I Info I Liks

Organisation I Staff I Research I Articles I Protocols & Facilities I NCMLS I Courses & Seminars I Info I Links